« Innovation for the study of tumor cell Proliferation in 3D »
We aim at investigating tumoral cell dynamics and proliferation in an integrated context using original multicellular tumor spheroid 3D models (MCTS), giving much attention to the systemic study of the biology of cell cycle control and cell fate. We study cell cycle and checkpoint control in response to physical, chemical and microenvironnemental cues, and search for 3D specific regulatory mechanisms. This project relies on developing original genetically engineered MCTS models and on the design of innovative experimental strategies using microdevices. Our work also relies on developing 3D imaging Light Sheet Fluorescence Microscopy (SPIM). We are conducting developments on the adaptive optics, on the implementation of photomanipulation devices in the optical path, and on the engineering of innovative and versatile sample holders. Mathematical modeling and computational simulation of in virtuo 3D growth are developed and feed with collected experimental data. These innovations are implemented to study the 3D response to genotoxic injuries and develop innovative models for pharmacological evaluation.
Project Managers: Valérie LOBJOIS (MCU), Corinne LORENZO (IR)
Odile MONDESERT (IE), Martine CAZALES (AI), Aurélie GOMES (AI), Aurore MASSON (PostDoc ANR), Claire JARDET (PhD CIFRE), Sophie ABADIE (PhD CIFRE), Ludivine GUILLAUME (PhD), Fabien GAVA (PhD), Jorge ROYES (PostDoc), Lise RIGAL (IE), Adrien RUSSO (IE).
We expect that this work will open up various perspectives, particularly in the use of the microtissues and associated technologies developed in this project for the assessment of therapeutic efficacy and for the design of pharmacological applications.
Cancer Biology, 3D Imaging, Multi cellular tumor Spheroid, Optronics, Light sheet microscopy (SPIM)
Cooperations, partnerships and valorisation
- Katia GRENIER (LAAS, Toulouse) – Project funded by ANR-DGA
- Gladys Mirey (INRA, Toulouse) - Project funded by ANR
- VORTEX team (IRIT, Toulouse) – Project funded by IDEX
- Stefan Chassaing (ITAV)
- Pablo LOZA (ICFO Barcelona, Spain)
- Pierre WEISS (ITAV)
- Collaboration with the company IMACTIV-3D (Toulouse)
- Collaboration with the company STANDA (Caen)
- Toulouse Tech Transfer (SATT Toulouse-Midi-Pyrénées)