« Innovation for the study of tumor cell Proliferation in 3D »
Valérie LOBJOIS & Bernard DUCOMMUN
Therapeutic options for cancer have considerably improved over the last decades thanks to a better understanding of cancer cell biology. However, most studies are often carried out using classical monolayer cell culture models that do not take into account essential parameters of a 3D organized tumor such as cellular heterogeneity, cell-cell and cell-microenvironment interactions. The aim of our team project is to investigate tumor cell dynamics and proliferation using in vitro microtumor models known as multicellular tumor spheroid 3D (MCTS). We study cell cycle and checkpoint control in response to physical, chemical and microenvironmental cues, and search for 3D specific regulatory mechanisms. Our work strongly relies on original genetically engineered MCTS expressing fluorescent reporters and biomarkers that are accessible to innovative 3D microscopy strategies both on live or fixed samples. These innovations are implemented to explore the dynamics of the response to anticancer drugs and to develop innovative models for pharmacological evaluation of new therapeutic agents.
Bernard DUCOMMUN (PUPH), Valérie LOBJOIS (MCU), Odile MONDESERT (IE), Aurélie GOMES (IE CDD), Claire JARDET (PhD student CIFRE), Ludivine GUILLAUME (PhD student), Fabien GAVA (PhD student), Lise RIGAL (IE CDD).
We expect this work will open various perspectives, particularly in the use of the MCTS and associated technologies developed in this project for the assessment of therapeutic efficacy and for the design of pharmacological applications.
Cancer Biology, Cell cycle, proliferation, Multi cellular tumor Spheroid
Cooperations, partnerships and valorisation
- Collaboration with IMACTIV-3D (Centre Pierre Potier)
- Collaboration with Genoskin (Centre Pierre Potier)
- Collaboration VORTEX team (Institut de Recherche en Informa-tique de Toulouse) and ONKO3D team (ITAV)
- Katia GRENIER (LAAS, Toulouse) – Project funded by ANR-DGA
- Gladys Mirey (INRA, Toulouse) – Project funded by ANR
- Stefan Chassaing (ITAV)
- Toulouse Tech Transfer (SATT Toulouse-Midi-Pyrénées)